1. Antibodies
  2. Homo sapiens
  3. AGO1

ENCAB189GDE

Antibody against Homo sapiens AGO1

Homo sapiens
K562, HepG2
characterized to standards
Status
released
Source (vendor)
Cell Signaling
Product ID
5053
Lot ID
1
Characterized targets
AGO1 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Isotype
IgG
Antigen description
A synthetic peptide corresponding to the sequence around Ile392 of human Argonaute 1 protein
Aliases
xiang-dong-fu:EIF2C1
External resources

Characterizations

AGO1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using AGO1/EIF2C1 specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit monoclonal anti-AGO1/EIF2C11 antibody (lanes under 'anti-AGO1'). Asterisk indicates heavy chain of antibody.
Submitted by
Marcus Ho
Lab
Xiang-Dong Fu, UCSD
Download
 AGO1_HepG2.png
AGO1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using AGO1/EIF2C1 specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit monoclonal anti-AGO1/EIF2C11 antibody (lanes under 'anti-AGO1'). Asterisk indicates heavy chain of antibody.
Submitted by
Marcus Ho
Lab
Xiang-Dong Fu, UCSD
Download
 AGO1_K562.png
AGO1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following shRNA against EIF2C1 in HepG2 whole cell lysate using EIF2C1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF2C1. EIF2C1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn