1. Antibodies
  2. Homo sapiens
  3. PLRG1

ENCAB182IKE

Antibody against Homo sapiens PLRG1

Homo sapiens
HepG2
characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-940A
Lot ID
1
Characterized targets
PLRG1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1077
External resources

Characterizations

PLRG1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A301-940A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
PLRG1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line HepG2 using the antibody A301-940A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 57.194.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
PLRG1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from HepG2 nuclear cell lysates using the antibody A301-940A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
PRPF19 and PLRG1 are components of some complexs, http://www.genecards.org/cgi-bin/carddisp.pl?gene=PRPF19&keywords=prpf19. HNRNPA3 involves RNA binding and mRNA splicing.
Reviewer comment
PLRG1 is not the top ranked protein but is the top ranked TF by enrichment.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford