ENCAB180KRZ
Antibody against Homo sapiens SAFB
Homo sapiens
HepG2, K562
characterized to standards
- Status
- released
- Source (vendor)
- Zen Bioscience
- Product ID
- #200580
- Lot ID
- 20130304
- Characterized targets
- SAFB (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Isotype
- IgG1
- Antigen description
- Purified recombinant human SAFB protein fragments expressed in E.coli.
- Aliases
- xiang-dong-fu:SAFB
- External resources
Characterizations
SAFB (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using SAFB specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SAFB antibody (lanes under 'SAFB').
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
SAFB (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using SAFB specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SAFB antibody (lanes under 'SAFB').
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
SAFB (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against SAFBSAFB specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SAFB.SAFB protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- SAFB-CRISPR-K562.png
SAFB (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against SAFB in HepG2 whole cell lysate using SAFB specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SAFB.SAFB protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889
- Download
- SAFB-HEPG2-CRISPR.png
SAFB (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using SAFB specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-SAFB antibody (lanes under 'anti-SAFB'). Asterisk indicates light chain of antibody.
- Submitted by
- Marcus Ho
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- SAFB_HepG2.png
SAFB (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using SAFB specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-SAFB antibody (lanes under 'anti-SAFB').Asterisk indicates light chain of antibody.
- Submitted by
- Marcus Ho
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- SAFB_K562.png