ENCAB179WCQ
Antibody against Homo sapiens AGO1
Homo sapiens
K562
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- MBLI
- Product ID
- RN028PW
- Lot ID
- 002
- Characterized targets
- AGO1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Peptide, N-terminus of human EIF2C1
- External resources
Characterizations
AGO1 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against AGO1/EIF2C1 in HepG2 whole cell lysate using EIF2C1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF2C1. EIF2C1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- EIF2C1-HEPG2-RN028PW.png
AGO1 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against EIF2C1 in K562 whole cell lysate using EIF2C1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF2C1. EIF2C1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- EIF2C1-k562-RN028PW.png
AGO1 (Homo sapiens)
Method: knockdown or knockout
not submitted for review by lab
- Caption
- Western blot following shRNA against EIF2C1 in K562 whole cell lysate using EIF2C1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF2C1. EIF2C1 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- EIF2C1_Secondary_Western.png
AGO1 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-WB characterization of AGO1 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-EIF2C1(AGO1)(Human)pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- K562_MBLI_RN028PW_002_EIF2C1.png
AGO1 (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB characterization of AGO1 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-EIF2C1(AGO1)(Human)pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN028P_001_EIF2C1.png