1. Antibodies
  2. Homo sapiens
  3. HDGF

ENCAB173GGB

Antibody against Homo sapiens HDGF

Homo sapiens
MCF-7, HepG2
characterized to standards
Homo sapiens
K562, GM12878, HEK293T
characterized to standards with exemption
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-170A
Lot ID
1
Characterized targets
HDGF (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
External resources

Characterizations

HDGF (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A303-170A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Molecular weight: 26788 Da
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
HDGF (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A303-170A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected Molecular Weight: 28 KD
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
HDGF (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A303-170A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitter comment
HDGF is known to form a domain-swapped homodimer that can be stable even in reducing gels (PMID: 17270212)
Reviewer comment
Higher than expected size and multiple bands were noted. In response to the submitter's comment, the stable domain-swapped homodimer would explain the higher (double-sized) band and also explain why the ratio of the two bands can be different on different westerns.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
 6.jpg
HDGF (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
IP-WB analysis of K562 whole cell lysate using HDGF specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal HDGF Antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
HDGF (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody A303-170A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 26.788.
Submitter comment
HDGF is known to form a domain-swapped homodimer that can be stable even in reducing gels (PMID: 17270212)
Reviewer comment
Higher than expected size and multiple bands were noted. In response to the submitter's comment, the stable domain-swapped homodimer would explain the higher (double-sized) band and also explain why the ratio of the two bands can be different on different westerns.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
HDGF (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody A303-170A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 26.788.
Submitter comment
HDGF is known to form a domain-swapped homodimer that can be stable even in reducing gels (PMID: 17270212)
Reviewer comment
Higher than expected size and multiple bands were noted. In response to the submitter's comment, the stable domain-swapped homodimer would explain the higher (double-sized) band and also explain why the ratio of the two bands can be different on different westerns.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
HDGF (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following shRNA against HDGF in HepG2 whole cell lysate using HDGF specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HDGF.HDGF protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
HDGF (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following shRNA against HDGF in K562 whole cell lysate using HDGF specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against HDGF.HDGF protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
Download
 HDGF-K562.png