ENCAB171LTJ
Antibody against Homo sapiens NONO
Homo sapiens
HepG2
partially characterized
Homo sapiens
at least one cell type or tissue
not pursued
- Status
- released
- Source (vendor)
- MBLI
- Product ID
- RN092PW
- Lot ID
- 001
- Characterized targets
- NONO (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Peptide, N-terminus of human NONO
- Aliases
- xiang-dong-fu:NONO
- External resources
Characterizations
NONO (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using NONO specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-NONO(P54NRB)pAb. This antibody did not meet our primary validation criteria using our standard IP protocol in the indicated cell type.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN092PW_001_NONO.png
NONO (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using NONO specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit polyclonal anti-NONO antibody (lanes under 'anti-NONO'). Asterisk indicates heavy chain of antibody.
- Submitted by
- Marcus Ho
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- NONO_HepG2_MBL.png