1. Antibodies
  2. Homo sapiens
  3. HDAC2

ENCAB170GTK

Antibody against Homo sapiens HDAC2

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Active Motif
Product ID
39533
Lot ID
10108002
Characterized targets
HDAC2 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Aliases
michael-snyder:AS-807
External resources

Characterizations

HDAC2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody 39533. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Band shared by IgG lane.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
HDAC2 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody 39533, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
HNRNPH1 and HNRNPH2 are involved in mRNA processing and mRNA splicing
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
HDAC2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody 39533. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 55.364.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford