ENCAB169CDD
Antibody against Homo sapiens H3K27me3
Homo sapiens
any cell type or tissue
characterized to standards
- Status
- released
- Source (vendor)
- Diagenode
- Product ID
- C15410069-10
- Lot ID
- A1821_D
- Characterized targets
- H3K27me3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Aliases
- bradley-bernstein:PchAb 1383
- External resources
Characterizations
H3K27me3 (Homo sapiens)
HeLa-S3
compliant
- Caption
- We performed a WB using 20ug of HeLa Nuclear Lysate. Lysate was resolved by electrophoresis on a 4-12% bis-tris gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Band of expected size (~17kDa) visualized in HeLa nuclear extract and cannot be detected in unmodified recombinant Histone H3. Antibody seems to be specific since no reactivity with non histone proteins or unmodified histones was detected.
- Reviewer comment
- Recombinant histone H3 is not included in the western blot but is included in the secondary dot blot, as allowed by the ENCODE antibody characterization standards.
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- UM1HG009390
H3K27me3 (Homo sapiens)
compliant
- Caption
- We performed a DB analysis using the large set of modified peptides provided in the Active Motif MODified Histone Peptide Array, catalog #13005. The only peptide recognized by the H3K27me3 antibody was the correct one.
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- UM1HG009390