ENCAB155VEG

Antibody against Homo sapiens H3K27me3

Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Cell Signaling
Product ID
9733S
Lot ID
10
Characterized targets
H3K27me3 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Isotype
IgG
Antigen description
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys27 is tri-methylated.
Aliases
bradley-bernstein:PchAb 1034
External resources

Characterizations

H3K27me3 (Homo sapiens)
K562
Method: immunoblot
compliant
Caption
0.7ug unmodified Recombinant Histone H3 (Abcam ab1791) and nuclear extract from 10ug K562 cells and 0.5ug of Recombinant Histone H3 were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4 C. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Panel A: anti Histone H3 (Abcam ab1791), band of expected size (~17kDa) visualized in both unmodified recombinant histone H3 and NL, at similar intensities. Panel B: Band of expected size (~17kDa) visualized in K562 nuclear lysates, and cannot be detected in unmodified recombinant Histone H3.
Submitted by
Kathrina Onate
Lab
Bradley Bernstein, Broad
H3K27me3 (Homo sapiens)
Method: dot blot assay
Attachment from submitter
compliant
Caption
Performed dot blot analysis using a large set of modified peptides. The only peptide recognized by the H3K27me3 antibody was the correct one.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad