ENCAB155VEG
Alternate accession: ENCAB760OMS
Antibody against Homo sapiens H3K27me3
Homo sapiens
any cell type or tissue
characterized to standards
- Status
- released
- Source (vendor)
- Cell Signaling
- Product ID
- 9733S
- Lot ID
- 10
- Characterized targets
- H3K27me3 (Homo sapiens)
- Host
- rabbit
- Clonality
- monoclonal
- Isotype
- IgG
- Antigen description
- Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys27 is tri-methylated.
- Aliases
- bradley-bernstein:PchAb 1034, bradley-bernstein:PchAb 1366
- External resources
Characterizations
H3K27me3 (Homo sapiens)
K562
compliant
- Caption
- 0.7ug unmodified Recombinant Histone H3 (Abcam ab1791) and nuclear extract from 10ug K562 cells and 0.5ug of Recombinant Histone H3 were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4 C. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Panel A: anti Histone H3 (Abcam ab1791), band of expected size (~17kDa) visualized in both unmodified recombinant histone H3 and NL, at similar intensities. Panel B: Band of expected size (~17kDa) visualized in K562 nuclear lysates, and cannot be detected in unmodified recombinant Histone H3.
- Submitted by
- Kathrina Onate
- Lab
- Bradley Bernstein, Broad
- Grant
- UM1HG009390
H3K27me3 (Homo sapiens)
compliant
- Caption
- Performed dot blot analysis using a large set of modified peptides. The only peptide recognized by the H3K27me3 antibody was the correct one.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- H3K27me3_CST_C36B11_lot10.png