1. Antibodies
  2. Homo sapiens
  3. ZNF687

ENCAB152GWU

Antibody against Homo sapiens ZNF687

Homo sapiens
MCF-7, K562
characterized to standards with exemption
Homo sapiens
HepG2, GM12878
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-278A
Lot ID
1
Characterized targets
ZNF687 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-987
External resources

Characterizations

ZNF687 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A303-278A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ZNF687 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody A303-278A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 129.529.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 8.jpg
ZNF687 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody A303-278A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 129.529.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZNF687 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A303-278A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 129.529.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZNF687 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
exempt from standards
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A303-278A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
OGDH is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. ZMYND8 has interaction with ZNF687, http://thebiogrid.org/121643/summary/homo-sapiens/znf687.html. And ZMYND8 was also detected in MS of ZNF687 (ENCAB146FZU). Both HNRNPUL1 and ZNF687 have interactions with EED (50KD) and RNF2 (38KD), http://thebiogrid.org/121643/summary/homo-sapiens/znf687.html and http://thebiogrid.org/116281/summary/homo-sapiens/hnrnpul1.html, EED and RNF2 are not in the same gel slice with HNRNPUL1 and ZNF687. LARP1 involves RNA binding and RNA translation activator.
Reviewer comment
Even though ZMYND8 is ranked higher than ZNF687, the submitter has noted that it has an interaction with ZNF687.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford