ENCAB148GIT
Alternate accession: ENCAB027PGE
Antibody against Homo sapiens GFI1B
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
GM12878, HepG2, K562, HeLa-S3, IMR-90, heart
not characterized to standards
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-28356
- Lot ID
- A1905
- Characterized targets
- GFI1B (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Isotype
- IgG2b
- Antigen description
- raised against amino acids 21-170 of Gfi-1B of human origin
- Aliases
- michael-snyder:AS-337, michael-snyder:GFI1B-human_sc-28356_A1905
- External resources
Characterizations
GFI1B (Homo sapiens)
compliant
- Caption
- The motif for target GFI1B is represented by the attached position weight matrix (PWM) derived from ENCFF141DDJ. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.86361426713 Global enrichment Z-score: 3.56613989922 Positional bias Z-score: 5.39586000428 Peak rank bias Z-score: 3.89281701547 Enrichment rank: 2.0
- Submitted by
- Kathrina Onate
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
GFI1B (Homo sapiens)
GM12878
not compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody sc-28356x. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Reviewer comment
- Indicated band not at least 50% of signal in IP lane.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
GFI1B (Homo sapiens)
not submitted for review by lab
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-28356x. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 37.492
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- expt1066-6-Gfi1B (B-7)-sc-28356x.jpg
GFI1B (Homo sapiens)
HepG2
not compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody sc-28356x. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 37.492
- Reviewer comment
- Multiple bands, some non-specific that have stronger signal than the marked band.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- Gfi1B (ENCAB148GIT)_HepG2.jpg
GFI1B (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90heart
not compliant
- Caption
- Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HeLaS3, HepG2, IMR90, Heart using the antibody sc-28356x. Molecular Weight: 37.492
- Submitter comment
- Multiple bands appear because GFI1B has two isoforms. Isoform 1 (Uniprot: [Q5VTD9-1]) is 37kDa; Isoform 2 (Uniprot: [Q5VTD9-2]) is 32kDa.
- Reviewer comment
- Multiple immunoreactive bands seen in all tested cell types. Higher than expected sized bands not accounted for by isoform information supplied by the submitter
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- GFL1B-WB-405.jpg.jpg