ENCAB148GIT

Alternate accession: ENCAB027PGE

Antibody against Homo sapiens GFI1B

Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
GM12878, IMR-90, HepG2, HeLa-S3, K562, heart
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-28356
Lot ID
A1905
Characterized targets
GFI1B (Homo sapiens)
Host
mouse
Clonality
monoclonal
Isotype
IgG2b
Antigen description
raised against amino acids 21-170 of Gfi-1B of human origin
Aliases
michael-snyder:AS-337, michael-snyder:GFI1B-human_sc-28356_A1905
External resources

Characterizations

GFI1B (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target GFI1B is represented by the attached position weight matrix (PWM) derived from ENCFF141DDJ. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.86361426713 Global enrichment Z-score: 3.56613989922 Positional bias Z-score: 5.39586000428 Peak rank bias Z-score: 3.89281701547 Enrichment rank: 2.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
GFI1B (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody sc-28356x. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Indicated band not at least 50% of signal in IP lane.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
GFI1B (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-28356x. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 37.492
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
GFI1B (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody sc-28356x. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 37.492
Reviewer comment
Multiple bands, some non-specific that have stronger signal than the marked band.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
GFI1B (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90heart
Method: immunoblot
Attachment from submitter
not compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HeLaS3, HepG2, IMR90, Heart using the antibody sc-28356x. Molecular Weight: 37.492
Submitter comment
Multiple bands appear because GFI1B has two isoforms. Isoform 1 (Uniprot: [Q5VTD9-1]) is 37kDa; Isoform 2 (Uniprot: [Q5VTD9-2]) is 32kDa.
Reviewer comment
Multiple immunoreactive bands seen in all tested cell types. Higher than expected sized bands not accounted for by isoform information supplied by the submitter
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford