1. Antibodies
  2. Homo sapiens
  3. IKZF1

ENCAB144WPX

Antibody against Homo sapiens IKZF1

Homo sapiens
K562, GM12878, HepG2
characterized to standards
Status
released
Source (vendor)
GeneTex
Product ID
GTX129438
Lot ID
41556
Characterized targets
IKZF1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1217
External resources

Characterizations

IKZF1 (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target IKZF1 is represented by the attached position weight matrix (PWM) derived from ENCFF321MEV. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.69170985 Global enrichment Z-score: 2.415727275 Positional bias Z-score: 1.793387109 Peak rank bias Z-score: 2.134421415 Enrichment rank: 6.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
IKZF1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody GTX129438. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Molecular Weight: 57.528
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
 expt1042_5.jpg
IKZF1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody GTX129438. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 57.528
Reviewer comment
The arrow/mark in the IgG lane obscures where a band of the expected size could be.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
IKZF1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody GTX129438. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 57.528
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 HepG2-IKZF1.jpg
IKZF1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of IKZF1 from K562 cells using GTX129438. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with GTX129438. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. The expected band size is 57.5 kDa.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download
 IKZF1.jpeg
IKZF1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using GTX129438, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford