ENCAB135MQH
Antibody against Homo sapiens SMN1
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- MBLI
- Product ID
- RN077PW
- Lot ID
- 001
- Characterized targets
- SMN1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Peptide, cytoplasmic region of human SMN1
- External resources
Characterizations
SMN1 (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using SMN1 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-SMN1pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation. Secondary validation is necessary becuase the predicted band in not recognized in the 'input/supernatant' sample due to low expression level.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN077PW_001_SMN1.png
SMN1 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Wesrn blot following shRNA against SMN1 in K562 and HepG2 whole cell lysate using SMN1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SMN1. Lanes 5-8 follow the same pattern, but in HepG2. SMN1 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- SMN1_Secondary_Western.png