ENCAB126OWL
Antibody against Homo sapiens AGO2
Homo sapiens
K562, HepG2
characterized to standards
- Status
- released
- Source (vendor)
- Abnova
- Product ID
- H00027161-M01
- Lot ID
- E8251-S2
- Characterized targets
- AGO2 (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Isotype
- IgG1κ
- Antigen description
- EIF2C2 (AAH07633.1, 1 a.a. ~ 377 a.a) full-length recombinant protein with GST tag
- Antigen sequence
- MPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA
- Aliases
- xiang-dong-fu:EIF2C2
- External resources
Characterizations
AGO2 (Homo sapiens)
compliant
- Caption
- Western blot analysis following 2 siRNAs against AGO2 in HepG2 whole cell lysate using AGO2 specific antibody. Lane 1 is a ladder; lane 2-4 are 1/4, 1/2 and 100% of HepG2 non-targeting control of knockdown, respectively; lane 5 and 6 are two different siRNAs against AGO2. AGO2 protein appears as the green arrow, beta-actin serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- AGO2-HEPG2_FU's.png
AGO2 (Homo sapiens)
HepG2
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using AGO2/EIF2C2 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-AGO2/EIF2C2 antibody (lanes under 'anti-AGO2'). Asterisk indicates heavy and light chains of antibody.
- Submitted by
- Marcus Ho
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- AGO2_HepG2.png
AGO2 (Homo sapiens)
K562
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using AGO2/EIF2C2 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-AGO2/EIF2C2 antibody (lanes under 'anti-AGO2'). Asterisk indicates heavy and light chains of antibody.
- Submitted by
- Marcus Ho
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- AGO2_K562.png