ENCAB126OWL

Antibody against Homo sapiens AGO2

Homo sapiens
K562, HepG2
characterized to standards
Status
released
Source (vendor)
Abnova
Product ID
H00027161-M01
Lot ID
E8251-S2
Characterized targets
AGO2 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Isotype
IgG1κ
Antigen description
EIF2C2 (AAH07633.1, 1 a.a. ~ 377 a.a) full-length recombinant protein with GST tag
Antigen sequence
MPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA
Aliases
xiang-dong-fu:EIF2C2
External resources

Characterizations

AGO2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot analysis following 2 siRNAs against AGO2 in HepG2 whole cell lysate using AGO2 specific antibody. Lane 1 is a ladder; lane 2-4 are 1/4, 1/2 and 100% of HepG2 non-targeting control of knockdown, respectively; lane 5 and 6 are two different siRNAs against AGO2. AGO2 protein appears as the green arrow, beta-actin serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Xiang-Dong Fu, UCSD
AGO2 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using AGO2/EIF2C2 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-AGO2/EIF2C2 antibody (lanes under 'anti-AGO2'). Asterisk indicates heavy and light chains of antibody.
Submitted by
Marcus Ho
Lab
Xiang-Dong Fu, UCSD
Download
AGO2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using AGO2/EIF2C2 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-AGO2/EIF2C2 antibody (lanes under 'anti-AGO2'). Asterisk indicates heavy and light chains of antibody.
Submitted by
Marcus Ho
Lab
Xiang-Dong Fu, UCSD
Download