ENCAB108MXA
Antibody against Homo sapiens IGF2BP1
Homo sapiens
HepG2, K562
characterized to standards
- Status
- released
- Source (vendor)
- MBLI
- Product ID
- RN007P
- Lot ID
- 003
- Characterized targets
- IGF2BP1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Isotype
- IgA
- Antigen description
- KLH-conjugated synthetic peptide KQQHQKGQSNQAQARRK i561-577 aa
- External resources
Characterizations
IGF2BP1 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-WB analysis of HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is 1% twenty million whole cell lysate input, lane 2 is 1% of the supernatant afer IP and Lane 3 is 10% of IP enrichment using rabbit polyclonal anti-IGF2BP1 Antibody. Lane 4 is 1% ten million whole cell lysate input and lane 5 is 25% IP enrichment using rabbit normal IgG (lanes under 'IgG').
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- HepG2_MBLI_RN007P_003_IGF2BP1.png
IGF2BP1 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against IGF2BP1 in K562 and HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against IGF2BP1. 5-8 follow the same pattern, but in HepG2. IGF2BP1 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- IGF2BP1_CRISPR.png
IGF2BP1 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against IGF2BP1 in K562 and HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against IGF2BP1. Lanes 5-8 follow the same pattern, but in HepG2. IGF2BP1 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- IGF2BP1_Secondary_Western.png
IGF2BP1 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is 2.5% of five million whole cell lysate input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using either rabbit polyclonal anti-IGF2BP1 antibody (lanes under 'IGF2BP1') or using rabbit normal IgG (lanes under 'IgG').
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- K562_MBLI_RN007P_003_IGF2BP1.png