ENCAB108MXA

Antibody against Homo sapiens IGF2BP1

Homo sapiens
K562, HepG2
characterized to standards
Status
released
Source (vendor)
MBLI
Product ID
RN007P
Lot ID
003
Characterized targets
IGF2BP1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgA
Antigen description
KLH-conjugated synthetic peptide KQQHQKGQSNQAQARRK i561-577 aa
External resources

Characterizations

IGF2BP1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-WB analysis of HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is 1% twenty million whole cell lysate input, lane 2 is 1% of the supernatant afer IP and Lane 3 is 10% of IP enrichment using rabbit polyclonal anti-IGF2BP1 Antibody. Lane 4 is 1% ten million whole cell lysate input and lane 5 is 25% IP enrichment using rabbit normal IgG (lanes under 'IgG').
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
IGF2BP1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following CRISPR against IGF2BP1 in K562 and HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against IGF2BP1. 5-8 follow the same pattern, but in HepG2. IGF2BP1 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
IGF2BP1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against IGF2BP1 in K562 and HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against IGF2BP1. Lanes 5-8 follow the same pattern, but in HepG2. IGF2BP1 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
IGF2BP1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is 2.5% of five million whole cell lysate input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using either rabbit polyclonal anti-IGF2BP1 antibody (lanes under 'IGF2BP1') or using rabbit normal IgG (lanes under 'IgG').
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD