ENCAB076QHX
Antibody against Homo sapiens SIRT6
Homo sapiens
GM12878, endothelial cell of umbilical vein
partially characterized
Homo sapiens
K562, HepG2
not characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A302-451A
- Lot ID
- 1
- Characterized targets
- SIRT6 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Antigen description
- sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6, sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
- Aliases
- bradley-bernstein:PchAb 1239
- External resources
Characterizations
SIRT6 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
compliant
- Caption
- Nuclear lysates from K562 (10ug), HepG2 (10ug), GM12878 (7ug), HUVEC (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using the iblot system. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min.the strongest band was detected around the expected size (~50kDa).
- Reviewer comment
- barely within 20% of expected size, 39 kDa
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- SIRT6_Bethyl_A302-451A-1_WB.png