ENCAB076QHX

Antibody against Homo sapiens SIRT6

Homo sapiens
GM12878, endothelial cell of umbilical vein
partially characterized
Homo sapiens
K562, HepG2
not characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A302-451A
Lot ID
1
Characterized targets
SIRT6 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Antigen description
sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6, sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
Aliases
bradley-bernstein:PchAb 1239
External resources

Characterizations

SIRT6 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (10ug), GM12878 (7ug), HUVEC (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using the iblot system. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min.the strongest band was detected around the expected size (~50kDa).
Reviewer comment
barely within 20% of expected size, 39 kDa
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad