ENCAB057RGG
Antibody against Homo sapiens TARDBP
Homo sapiens
K562, HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A303-223A
- Lot ID
- 1
- Characterized targets
- TARDBP (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- External resources
Characterizations
TARDBP (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB characterization of TARDBP specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal anti-TDP43 Antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- Bethyl_A303-223A_1_TARDBP.png
TARDBP (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using TDP43 (TARDBP) specific antibody. Lane 1 is 1% of twenty million whole cell lysate input; lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'lgG'); Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-TDP43 antibody (lanes under 'TDP43'). The expected molecular weight of TDP43 is 43 kDa.
- Submitted by
- Xintao Wei
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- HepG2_Bethyl_A303-223A_1_TDP43.png
TARDBP (Homo sapiens)
K562HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using TARDBP specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-TDP43 antibody (lanes under 'TARDBP').
- Submitter comment
- This antibody was given and exemption for K562. The data was clean and the pattern is reliable.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- K562_Bethyl_A303-223A_1_TARDBP.png
TARDBP (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against TARDBP in HepG2 whole cell lysate using TARDBP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TARDBP.TARDBP protein appears as the green band, GAPDH serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- TARDBP-hepg2.png
TARDBP (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against TARDBP in K562 whole cell lysate using TARDBP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TARDBP. TARDBP protein appears as the green band, GAPDH serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- TARDBP_Secondary_Western.png