ENCAB052SZV

Antibody against Homo sapiens HDAC1

Homo sapiens
GM12878, endothelial cell of umbilical vein, K562
partially characterized
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-6298
Lot ID
D0813
Characterized targets
HDAC1 (Homo sapiens)
Host
goat
Clonality
polyclonal
Antigen description
histone deacetylase
Aliases
bradley-bernstein:PchAb 1277
External resources

Characterizations

HDAC1 (Homo sapiens)
K562GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from K562 (10ug), GM12878 (10ug), HUVEC (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using the iblot system. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Band of expected size visualized representing strongest signal in the lane.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad