K562, GM12878, endothelial cell of umbilical vein
HDAC1 (Homo sapiens)
K562GM12878endothelial cell of umbilical vein
- Nuclear lysates from K562 (10ug), GM12878 (10ug), HUVEC (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using the iblot system. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Band of expected size visualized representing strongest signal in the lane.
- Submitted by
- Noam Shoresh
- Bradley Bernstein, Broad