ENCAB039KCI

Antibody against Homo sapiens NCBP2

Homo sapiens
K562, HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A302-553A
Lot ID
1
Characterized targets
NCBP2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
External resources

Characterizations

NCBP2 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
IP-WB analysis of K562 whole cell lysate using NCBP2 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Rabbitanti-NCBP2Antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
NCBP2 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using NCBP2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NCBP2 antibody (lanes under 'NCBP2').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
NCBP2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using NCBP2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NCBP2 antibody (lanes under 'NCBP2').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
NCBP2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against NCBP2 in K562 and HepG2 whole cell lysate using NCBP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against NCBP2. Lanes 5-7 follow the same pattern, but in HepG2. NCBP2 protein appears as the green band, GAPDH serves as a control and appears in red.
Submitted by
Brenton Graveley
Lab
Brenton Graveley, UConn