ENCAB038XFS
Antibody against Homo sapiens SRSF5
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- MBLI
- Product ID
- RN082PW
- Lot ID
- 001
- Characterized targets
- SRSF5 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Peptide, N-terminus of human SRSF5
- Aliases
- xiang-dong-fu:SRSF5
- External resources
Characterizations
SRSF5 (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of MCF7 whole cell lysate using SRSF5 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-SRSF5(SRP40)pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN082PW_001_SRSF5.png
SRSF5 (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB characterization of SRSF5 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-SRSF5(SRP40)pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN082PW_001_SRSF5_K562.png
SRSF5 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against SRSF5 in HepG2 whole cell lysate using SRSF5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SRSF5.SRSF5 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- SRSF5-HEPG2_Secondary_Western.png
SRSF5 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against SRSF5 in K562 whole cell lysate using SRSF5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRSF5. SRSF5 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889
- Download
- SRSF5-K562-CRISPR.png