1. Antibodies
  2. Homo sapiens
  3. E2F1

ENCAB037OHX

Antibody against Homo sapiens E2F1

Homo sapiens
HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
K562
not characterized to standards
Status
released
Source (vendor)
GeneTex
Product ID
GTX101235
Lot ID
39812
Characterized targets
E2F1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1209
External resources

Characterizations

E2F1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody GTX101235. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 46.920
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
E2F1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody GTX101235. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 46.920
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
E2F1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody GTX101235. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 46.920
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 E2F1.jpg
E2F1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from HepG2 nuclear cell lysates using the antibody GTX101235, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
None of the proteins ranked above or equally ranked have been shown to be sequence-specific TFs (including TRIM27).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
E2F1 (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target E2F1 is represented by the attached position weight matrix (PWM) derived from ENCFF774WGM. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.799331189 Global enrichment Z-score: 3.695747681 Positional bias Z-score: 6.050255263 Peak rank bias Z-score: 6.211764869 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
E2F1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody GTX101235. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 46.920
Reviewer comment
Indicated band entirely overlapping signal detected in IgG lane at the same size (and is only major band in IP lane).
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
E2F1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody GTX101235. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 46.920
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
E2F1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line HepG2 using the antibody GTX101235. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 46.920.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford