ENCAB021IRD

Antibody against Homo sapiens YBX3

Homo sapiens
K562
characterized to standards
Homo sapiens
HepG2
characterized to standards with exemption
Homo sapiens
MCF-7, GM12878, any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-070A
Lot ID
1
Characterized targets
YBX3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
External resources

Characterizations

YBX3 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
IP-WB analysis of K562 whole cell lysate using YBX3 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal anti-ZONAB. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
YBX3 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody A303-070A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 40.090.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
YBX3 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A303-070A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 40.090
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
YBX3 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody A303-070A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 40.090.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
YBX3 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using ZONAB specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-ZONAB antibody (lanes under 'ZONAB').
Submitter comment
The expected size is 40kDa. In the submitted blot, the major immunoreactive band appears to be outside of the allowed 20% size deviation.
Reviewer comment
Though the major band is running higher than the expected size (>20% allowed size deviation), the accompanying knockdown secondary characterization shows that same sized band is specifically knocked down by siRNAs against YBX3.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
YBX3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using PRPF8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PRPF8 antibody (lanes under 'PRPF8').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
YBX3 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7 using the antibody A303-070A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 40.090.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
YBX3 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against YBX3 in K562 and HepG2 whole cell lysate using YBX3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against YBX3. Lanes 5-8 follow the same pattern, but in HepG2. YBX3 protein appears as the green band, GAPDH serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn