ENCAB014HCW
Antibody against Homo sapiens PPP1R10
Homo sapiens
GM12878, HEK293T
characterized to standards
Homo sapiens
K562
characterized to standards with exemption
Homo sapiens
MCF-7, HepG2
not characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A300-439A
- Lot ID
- 2
- Characterized targets
- PPP1R10 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- michael-snyder:AS-1558
- External resources
Characterizations
PPP1R10 (Homo sapiens)
MCF-7
not compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A300-439A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 99.058
- Reviewer comment
- Multiple bright bands
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1105_11_PNUTS_A300-439A.jpg
PPP1R10 (Homo sapiens)
HepG2
not compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A300-439A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 99.058
- Reviewer comment
- Band of interest is not 50% of overall signal in lane, and multiple bands appear in both input and the lane of interest.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1117_01_PNUTS_A300-439A.jpg
PPP1R10 (Homo sapiens)
K562
exempt from standards
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A300-439A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 99.058
- Submitter comment
- We analyzed the bands by IP-MS and confirmed that the factor of interest is detected.
- Reviewer comment
- There are multiple immunoreactive bands.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1117_02_PNUTS_A300-439A.jpg
PPP1R10 (Homo sapiens)
GM12878
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody A300-439A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 99.058
- Submitter comment
- We did 4 lanes as usual and the lane between IP and IgG is because the film shifted or IP lane was too full so 1 or 2 ul flew to the next lane.
- Reviewer comment
- Not sure about extra band on lane 4
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1120_01_PNUTS_A300-439A.jpg
PPP1R10 (Homo sapiens)
HEK293T
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody A300-439A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 99.058
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1120_02_PNUTS_A300-439A.jpg
PPP1R10 (Homo sapiens)
compliant
- Caption
- IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A300-439A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
- Submitted by
- Jessika Adrian
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- PPP1R10_A300-439A final.pdf
PPP1R10 (Homo sapiens)
K562
exempt from standards
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A300-439A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 99kDa
- Submitter comment
- We confirmed the multiple bands seen in the IP via mass spec.
- Reviewer comment
- The TF of interest was detected in marked bands A, B and C by mass spectrometry.
- Submitted by
- Jessika Adrian
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- PPP1R10_A300-439A.jpeg