ENCAB012FUX

Antibody against Homo sapiens BCLAF1

Homo sapiens
HepG2
characterized to standards
Homo sapiens
K562, GM12878, HEK293T, MCF-7
characterized to standards with exemption
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A300-608A
Lot ID
1
Characterized targets
BCLAF1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
External resources

Characterizations

BCLAF1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562 using the antibody A300-608A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 106.122.
Submitter comment
We think the signal runs higher than at the expected size possibly because BCLF1 can have binary interactions with BCL2 (~22-29kD) and EMD (~28kD): http://www.uniprot.org/uniprot/Q9NYF8#interaction. See also ENCAB733KID.
Reviewer comment
Not within 20% of expected size
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
BCLAF1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A300-608A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 106.122
Submitter comment
We think the signal runs higher than at the expected size possibly because BCLF1 can have binary interactions with BCL2 (~22-29kD) and EMD (~28kD): http://www.uniprot.org/uniprot/Q9NYF8#interaction. See also ENCAB733KID.
Reviewer comment
Not within 20% of expected size
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
BCLAF1 (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody A300-608A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 106.122
Submitter comment
We think the signal runs higher than at the expected size possibly because BCLF1 can have binary interactions with BCL2 (~22-29kD) and EMD (~28kD): http://www.uniprot.org/uniprot/Q9NYF8#interaction. See also ENCAB733KID.
Reviewer comment
Not within 20% of expected size
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
BCLAF1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody A300-608A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 106.122
Submitter comment
We think the signal runs higher than at the expected size possibly because BCLF1 can have binary interactions with BCL2 (~22-29kD) and EMD (~28kD): http://www.uniprot.org/uniprot/Q9NYF8#interaction. See also ENCAB733KID.
Reviewer comment
Not within 20% of expected size.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
BCLAF1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A300-608A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 106.122
Submitter comment
We think the signal runs higher than at the expected size possibly because BCLF1 can have binary interactions with BCL2 (~22-29kD) and EMD (~28kD): http://www.uniprot.org/uniprot/Q9NYF8#interaction. See also ENCAB733KID.
Reviewer comment
Not within 20% of expected size
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
BCLAF1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against BCLAF1 in K562 and HepG2 whole cell lysate using BCLAF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against BCLAF1. Lanes 5-8 follow the same pattern, but in HepG2. BCLAF1 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
BCLAF1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
IP-WB analysis of K562 whole cell lysate using BCLAF1 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal anti-BTF antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
BCLAF1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using BCLAF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-BCLAF1 antibody (lanes under 'BCLAF1').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD