1. Antibodies
  2. Homo sapiens
  3. IKZF2

ENCAB012FNR

Antibody against Homo sapiens IKZF2

Homo sapiens
GM12878, MCF-7
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-619A
Lot ID
1
Characterized targets
IKZF2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1548
External resources

Characterizations

IKZF2 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody A303-619A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 59.574
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
IKZF2 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A303-619A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 59.574
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
IKZF2 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody A303-619A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 59.574
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
IKZF2 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from GM12878 nuclear cell lysates using the antibody A303-619A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
The higher ranked TF IKZF3 has been shown to interacht with IKZF2 (https://thebiogrid.org/116485/summary/homo-sapiens/ikzf2.html).
Reviewer comment
Looks to be the first enriched TF in the list
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
IKZF2 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line GM12878 using the antibody A303-619A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 59.574.
Submitter comment
Looks like there is a primary band of the expected size
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford