ENCAB005NAZ

Antibody against Homo sapiens CCAR2

Homo sapiens
K562, HepG2
characterized to standards
Status
released
Source (vendor)
Zen Bioscience
Product ID
200093
Lot ID
AD0810
Characterized targets
CCAR2 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
affinity
Isotype
IgG1
Antigen description
Purified recombinant human DBC1 protein fragments expressed in E.coli
Aliases
xiang-dong-fu:DBC1
External resources

Characterizations

CCAR2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against DBC1 in HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against DBC1. DBC1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
CCAR2 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-DBC1 antibody (lanes under 'DBC1'). Asterisk indicates heavy and light chains of antibody.
Submitted by
Marcus Ho
Lab
Xiang-Dong Fu, UCSD
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CCAR2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using DBC1 specific antibody. Lane 1 is 4% of ten million whole cell lysate input (lane under '20% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-DBC1 antibody (lanes under 'anti-DBC1').
Submitter comment
genecards says Mass (Da): 102,902
Submitted by
Rui Xiao
Lab
Xiang-Dong Fu, UCSD
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