ENCAB005NAZ
Antibody against Homo sapiens CCAR2
Homo sapiens
HepG2, K562
characterized to standards
- Status
- released
- Source (vendor)
- Zen Bioscience
- Product ID
- 200093
- Lot ID
- AD0810
- Characterized targets
- CCAR2 (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Purification
- affinity
- Isotype
- IgG1
- Antigen description
- Purified recombinant human DBC1 protein fragments expressed in E.coli
- Aliases
- xiang-dong-fu:DBC1
- External resources
Characterizations
CCAR2 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against DBC1 in HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against DBC1. DBC1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- DBC1-HEPG2_FU's.png
CCAR2 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-DBC1 antibody (lanes under 'DBC1'). Asterisk indicates heavy and light chains of antibody.
- Submitted by
- Marcus Ho
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- DBC1_HepG2.png
CCAR2 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using DBC1 specific antibody. Lane 1 is 4% of ten million whole cell lysate input (lane under '20% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-DBC1 antibody (lanes under 'anti-DBC1').
- Submitter comment
- genecards says Mass (Da): 102,902
- Submitted by
- Rui Xiao
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- DBC1_K562.png