ENCAB000GBD

Antibody against Homo sapiens ZBTB7B

Homo sapiens
MCF-7, K562, HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
HEK293T
not characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-701A
Lot ID
1
Characterized targets
ZBTB7B (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1045
External resources

Characterizations

ZBTB7B (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A303-701A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 58.027
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZBTB7B (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A303-701A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 58.027
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZBTB7B (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody A303-701A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 58.027
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZBTB7B (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target ZBTB7B is represented by the attached position weight matrix (PWM) derived from ENCFF070WUF. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.681678279217 Global enrichment Z-score: -0.152665158197 Positional bias Z-score: 1.59322956416 Peak rank bias Z-score: 1.66599203246 4 Enrichment rank: 1.0
Submitted by
Jessika Adrian
Lab
Michael Snyder, Stanford
ZBTB7B (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A303-701A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 58.027
Reviewer comment
Gel says 102 KD, Uniprot says 58 KD. Still not compliant, not 50% of signal
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ZBTB7B (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody A303-701A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 58.027
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZBTB7B (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A303-701A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 58.027.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford