ENCAB000BSJ

Antibody against Homo sapiens H3K4me3

Homo sapiens
any cell type or tissue
characterized to standards with exemption
Status
released
Source (vendor)
Abcam
Product ID
ab8580
Lot ID
GR95252
Characterized targets
H3K4me3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide corresponding to Human Histone H3 aa 1-100 (tri methyl K4) conjugated to Keyhole Limpet Haemocyanin (KLH).
External resources

Characterizations

H3K4me3 (Homo sapiens)
Method: dot blot assay
Attachment from submitter
compliant
Caption
We performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-667, http://www.millipore.com/catalogue/item/16-667#). The blot has histone H3 peptides. The antibody recognized the H3K4me3 peptide 100%. While the recombinant unmodified version of that histone shows a lack of reactivity.
Submitted by
Minyi Shi
Lab
Michael Snyder, Stanford
H3K4me3 (Homo sapiens)
K562GM19239GM19240
Method: immunoblot
Attachment from submitter
exempt from standards
Submitter comment
We are out of this antibody lot and cannot do a primary, we hope the dot blot we've done is sufficient.
Reviewer comment
This antibody characterization was exempted by the ENCODE antibody review panel on 08/23/16.
Submitted by
Esther Chan
Lab
J. Michael Cherry, Stanford