ENCAB000BSJ
Antibody against Homo sapiens H3K4me3
Homo sapiens
any cell type or tissue
characterized to standards with exemption
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab8580
- Lot ID
- GR95252
- Characterized targets
- H3K4me3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Synthetic peptide corresponding to Human Histone H3 aa 1-100 (tri methyl K4) conjugated to Keyhole Limpet Haemocyanin (KLH).
- External resources
Characterizations
H3K4me3 (Homo sapiens)
compliant
- Caption
- We performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-667, http://www.millipore.com/catalogue/item/16-667#). The blot has histone H3 peptides. The antibody recognized the H3K4me3 peptide 100%. While the recombinant unmodified version of that histone shows a lack of reactivity.
- Submitted by
- Minyi Shi
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- H3K4me3_dotblot.png
H3K4me3 (Homo sapiens)
K562GM19239GM19240
exempt from standards
- Submitter comment
- We are out of this antibody lot and cannot do a primary, we hope the dot blot we've done is sufficient.
- Reviewer comment
- This antibody characterization was exempted by the ENCODE antibody review panel on 08/23/16.
- Submitted by
- Esther Chan
- Lab
- J. Michael Cherry, Stanford
- Grant
- U41HG006992
- Download
- no_more_antibody.png