1. Antibodies
  2. Homo sapiens
  3. HIST2H3A + HIST2H3D + HIST2H3C

ENCAB000BSI

Antibody against Homo sapiens H3K4me2

Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab7766
Lot ID
GR99281
Characterized targets
H3K4me2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, di methylated at K4.
External resources

Characterizations

H3K4me2 (Homo sapiens)
K562GM12878
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: K562,GM12878 using the antibody ab7766. Molecular Weight: 17.
Reviewer comment
Non-reactivity of this antibody lot against unmodified H3 is demonstrated on the dot blot.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
H3K4me2 (Homo sapiens)
Method: dot blot assay
Attachment from submitter
Attachment from submitters
compliant
Caption
We performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-667, http://www.millipore.com/catalogue/item/16-667#). The blot has histone H3 peptides. The antibody recognized the H3K4me2 peptide 100%. While the recombinant unmodified version of that histone shows a lack of reactivity
Submitted by
Minyi Shi
Lab
Michael Snyder, Stanford