ENCAB000BSH
Antibody against Homo sapiens H3K4me1
Homo sapiens
any cell type or tissue
characterized to standards with exemption
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab8895
- Lot ID
- GR46387
- Characterized targets
- H3K4me1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, mono methylated at K4.
- External resources
Characterizations
H3K4me1 (Homo sapiens)
Method: ChIP-seq comparison
exempt from standards
- Submitter comment
- We were unable to run IDR to do the ChIP-seq comparison as stipulated given that the peaks come from ChIP-seq and ChIA-PET experiments respectively, we hope what we've done instead is sufficient.
- Reviewer comment
- This antibody characterization was exempted by the ENCODE antibody review panel on 08/23/16.
- Submitted by
- Esther Chan
- Lab
- J. Michael Cherry, Stanford
- Grant
- U41HG006992
- Download
- ChIPComparison_H3K4me1.pdf
H3K4me1 (Homo sapiens)
K562GM19239GM19238GM19240
Method: immunoblot
exempt from standards
- Submitter comment
- We are out of this antibody lot and cannot do a primary, we hope the ChIP-seq comparison we've done is sufficient.
- Reviewer comment
- This antibody characterization was exempted by the ENCODE antibody review panel on 08/23/16.
- Submitted by
- Esther Chan
- Lab
- J. Michael Cherry, Stanford
- Grant
- U41HG006992
- Download
- no_more_antibody.png