ENCAB000BRO
Antibody against Homo sapiens SIRT1
Homo sapiens
K562, HepG2
partially characterized
- Status
- released
- Source (vendor)
- Active Motif
- Product ID
- 39354
- Lot ID
- 22808001
- Characterized targets
- SIRT1 (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Purification
- Protein G
- Isotype
- IgG1
- Antigen description
- Raised against a synthetic peptide corresponding to amino acids 581-630 of human SIRT1
- External resources
Characterizations
SIRT1 (Homo sapiens)
K562HepG2
compliant
- Caption
- An immunoprecipitation followed by Western blot was performed with Active Motif 39354 (lot 22808001) antibody against SIRT1 in K562 and HepG2 whole cell lysate along with a control IP with normal IgG from the same host species as the test antibody (mouse, Santa Cruz sc-2025). The expected size of SIRT1 is 120 kDa. A band of ~100 kDa was detected in the test IP and not in the control IP. The experiment was repeated and this band was analyzed by mass spec in the secondary validation.
- Submitted by
- Flo Pauli-Behn
- Lab
- Richard Myers, HAIB
- Grant
- U54HG006998
- Download
- SIRT1_39354_22808001_IP-Western.png