1. Antibodies
  2. Homo sapiens
  3. PROX1

ENCAB000BRH

Antibody against Homo sapiens PROX1

Homo sapiens
HepG2
not characterized to standards
Status
released
Source (vendor)
Active Motif
Product ID
61092
Lot ID
14511001
Characterized targets
PROX1 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
Protein G
Isotype
IgG2b
Antigen description
Raised against a recombinant protein corresponding to amino acids 547-737 of human Prox1.
External resources

Characterizations

PROX1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
An immunoprecipitation followed by Western blot was performed with Active Motif 61092 (lot 14511001) antibody against PROX1 in HepG2 whole cell lysate along with a control IP with no lysate. The expected size of PROX1 is 83 kDa and a band of ~80 kDa was detected in both the IP and the control. However, the experiment was repeated and this band was analyzed by mass spec in the secondary validation and the antibody was confirmed to detect PROX1.
Submitter comment
band found in the control and no IgG control
Submitted by
Flo Pauli-Behn
Lab
Richard Myers, HAIB
PROX1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Whole cell lysates of HepG2 were immunoprecipitated using the primary antibody (Active Motif; 61092). The IP fraction was separated on a 12% acrylamide gel with the Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. The membrane was probed with the primary antibody (same as that used for IP) and a secondary HRP-conjugated antibody. The resulting bands were visualized with SuperSignal West Femto Solution (Thermo Scientific). Protein Marker (PM) is labeled in kDa. The approximate size of PROX1 is ~90 kDa.
Reviewer comment
Band of interest is not 50% of overall signal in lane and is shared by the IgG control lane
Submitted by
Mark Mackiewicz
Lab
Richard Myers, HAIB