ENCAB000BPW

Antibody against Homo sapiens HIPK1

Homo sapiens
K562, HepG2
partially characterized
Status
released
Source (vendor)
Active Motif
Product ID
39726
Lot ID
3610001
Characterized targets
HIPK1 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
Protein G
Isotype
IgG
Antigen description
antibody was raised against a recombinant protein including amino acids 702-925 of mouse HIPK1
External resources

Characterizations

HIPK1 (Homo sapiens)
K562HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
An immunoprecipitation followed by Western blot was performed with Active Motif 39726 (lot03610001) antibody against HIPK1 in K562 and HEPG2 whole cell lysate along with a control IP with normal IgG from the same host species as the test antibody (mouse, Santa Cruz sc-2025). The expected size of HIPK1 is 131 kDa. Bands of ~45 and 170 kDa were detected in the test IP and not in the control IP. The experiment was repeated and these bands were analyzed by mass spec in the secondary validation.
Submitted by
Flo Pauli-Behn
Lab
Richard Myers, HAIB