ENCAB000BNO
Antibody against Homo sapiens SIX1
Homo sapiens
K562, GM12878, HepG2
partially characterized
Homo sapiens
HeLa-S3, MCF-7, liver
not characterized to standards
- Status
- released
- Source (vendor)
- Sigma
- Product ID
- HPA001893
- Lot ID
- C75776
- Characterized targets
- SIX1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Homeobox protein SIX1 recombinant protein epitope signature tag (PrEST)
- Antigen sequence
- CFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDS
- External resources
Characterizations
SIX1 (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
Method: immunoblot
compliant
- Caption
- Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HepG2, HelaS3, MCF7 and Liver using the antibody HPA001893. Molecular mass: 32210 Da
- Reviewer comment
- Band is either missing (lane 6) or is not 50% of signal (lanes 4 and 5). Lanes 1, 2, and 3 are compliant.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- SIX1_HPA001893_WB_a.jpg
SIX1 (Homo sapiens)
K562
Method: immunoprecipitation
not compliant
- Caption
- b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody HPA001893 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~32 kDa.
- Reviewer comment
- IgG control shares same band
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- SIX1_HPA001893_WB_b.jpg