ENCAB000BNK

Antibody against Homo sapiens UTY

Homo sapiens
at least one cell type or tissue
not pursued
Homo sapiens
GM12878, K562, HepG2, HeLa-S3, MCF-7, liver, HEK293T
not characterized to standards
Status
released
Source (vendor)
Sigma
Product ID
HPA001165
Lot ID
A01107
Characterized targets
UTY (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Ubiquitously transcribed Y chromosome tetratricopeptide repeat protein (Ubiquitously transcribed TPR protein on the Y chromosome).
Antigen sequence
SNSCILLDKCPPPRPPTSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPKNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWRCESNRSHTTI
External resources

Characterizations

UTY (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody HPA001165. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 149.5.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
UTY (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody HPA001165. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 149.5
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
UTY (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody HPA001165. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 149.5
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
UTY (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody HPA001165. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 149.5
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
UTY (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody HPA001165. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 149.5
Reviewer comment
The immunoreactive band runs way higher than expected (>allowed 20% deviation).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
UTY (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
Method: immunoblot
Attachment from submitter
not compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HepG2, HelaS3, MCF7 and Liver using the antibody HPA001165. Molecular mass: 149548 Da
Reviewer comment
Major band is at 250 kD rather than 150, and/or is not 50% of signal
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
UTY (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody HPA001165 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Strongest band is at 250, UniProt suggests 150KD
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford