ENCAB000BNE

Antibody against Homo sapiens IRF8

Homo sapiens
GM12878
partially characterized
Homo sapiens
K562, HepG2, HeLa-S3, MCF-7, liver
not characterized to standards
Status
released
Source (vendor)
Sigma
Product ID
HPA002267
Lot ID
A81887
Characterized targets
IRF8 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Interferon regulatory factor 8 recombinant protein epitope signature tag (PrEST)
Antigen sequence
QGVFVKRLCQGRVFCSGNAVVCKGRPNKLERDEVVQVFDTSQFFRELQQFYNSQGRLPDGRVVLCFGEEFPDMAPLRSKLILVQIEQLYVRQLAEEAGKSCGAGSVMQAPEEPPPDQVFRMFPDICASHQRSFFRENQQIT
External resources

Characterizations

IRF8 (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
Method: immunoblot
Attachment from submitter
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HepG2, HelaS3, MCF7 and Liver using the antibody HPA002267. Molecular mass: 48356 Da
Reviewer comment
Band is not 50% of overall signal (Lanes 2, 3, 4, 5, 6). Lane 1 is compliant.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
IRF8 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody HPA002267 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size is ~48 kDa.
Reviewer comment
IgG control shares same band
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford