1. Antibodies
  2. Homo sapiens
  3. MTA3

ENCAB000BML

Antibody against Homo sapiens MTA3

Homo sapiens
K562, MCF-7, HEK293T
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
GM12878, HepG2, HeLa-S3, liver
not characterized to standards
Status
released
Source (vendor)
Sigma
Product ID
HPA039433
Lot ID
R36305
Characterized targets
MTA3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Metastasis associated 1 family, member 3 recombinant protein epitope signature tag (PrEST)
Antigen sequence
MPTQSEEEKLSPSPTTEDPRVRSHVSRQAMQGMPVRNTGSPKSAVKTRQA
External resources

Characterizations

MTA3 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody HPA039433. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
MTA3 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody HPA039433. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Multiple bands and marked band is not >50% of the total signal in the lane. May perhaps be rescued with siRNA knockdown.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
MTA3 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody HPA039433. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 67.5
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
 expt1035_5.jpg
MTA3 (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody HPA039433. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 67.5
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
MTA3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of MTA3 from K562 cells using HPA039433. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with HPA039433. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. The expected band size is 67.5 kDa.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download
 MTA3.jpeg
MTA3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody HPA039433. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 67.5.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 MTA3.jpeg
MTA3 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using HPA039433, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
Both MTA3 and DHX9 have interaction with AURKA, and DHX9 has interaction with HNRNPR and HNRNPC. http://thebiogrid.org/121568/summary/homo-sapiens/mta3.html http://thebiogrid.org/108025/summary/homo-sapiens/dhx9.html
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
MTA3 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody HPA039433, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
None of WTAP, FUS and DLST are DNA binding proteins
Reviewer comment
Highest ranking transcription factor
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
MTA3 (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
Method: immunoblot
Attachment from submitter
Attachment from submitters
not compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HepG2, HelaS3, MCF7 and Liver using the antibody HPA039433. Molecular mass: 67504 Da
Reviewer comment
Band is not 50% of overall signal
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
MTA3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody HPA039433 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford