ENCAB000BLK
Antibody against Homo sapiens NRF1
Homo sapiens
K562, HepG2, HeLa-S3, MCF-7
characterized to standards
Homo sapiens
GM12878, liver
not characterized to standards
- Status
- released
- Source (vendor)
- CDI
- Product ID
- R157.1.3D4
- Lot ID
- 011513.RAP
- Characterized targets
- NRF1 (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Purification
- affinity
- Isotype
- IgG2b
- External resources
Characterizations
NRF1 (Homo sapiens)
Method: motif enrichment
compliant
- Caption
- The motif for target NRF1 is represented by the attached position weight matrix (PWM) derived from file ENCFF459SVS. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.919608216391, Global enrichment Z-score: 6.18745030991, Positional bias Z-score: 13.5938045651, Peak rank bias Z-score: 14.9932455973, Enrichment rank: 1.0.
- Submitted by
- Aditi Narayanan
- Lab
- Richard Myers, HAIB
- Grant
- U54HG006998
- Download
- ENCAB000BLK.pdf
NRF1 (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
Method: immunoblot
compliant
- Caption
- Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HeLaS3, MCF-7, Liver using the antibody R157.1.3D4. Molecular Weight: 57.0
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- NRF1_6lines.jpg
NRF1 (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
Method: immunoblot
not compliant
- Caption
- a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3, MCF7, Liver using the antibody R157.1.3D4.
- Reviewer comment
- There's 5 lanes but 6 are labeled for review. Multiple bands, blob near expected size of 53kD/46kD (not indicated on blot or caption).
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- NRF1_R157.1.3D4_WB_a.jpg
NRF1 (Homo sapiens)
K562
Method: immunoprecipitation
not compliant
- Caption
- b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562 , using the antibody R157.1.3D4 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~53 kDa.
- Reviewer comment
- Band not within 20% of expected size
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- NRF1_R157.1.3D4_WB_b.jpg