ENCAB000BLE

Antibody against Homo sapiens H3K4me3

Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Millipore
Product ID
07-473
Lot ID
DAM1623866
Characterized targets
H3K4me3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Aliases
bradley-bernstein:PchAb 99-V
External resources

Characterizations

H3K4me3 (Homo sapiens)
K562
Method: immunoblot
Attachment from submitter
compliant
Caption
Western blot analysis of K562 lysate using rabbit polyclonal to H3K4me3. Submitting lab has confirmed with the vendor that lots R0903B0054, DAM1619901, DAM1623866, DAM1644057, DAM1651667, and DAM1661080 are identical.
Submitter comment
M Hatan reports lab confirmed with vendor that these lots are identical and should require only one characterization: DAM1619901, DAM1623866, DAM1644057, DAM1651667, DAM1661080, R0903B0055
Submitted by
Meital Hatan
Lab
Bradley Bernstein, Broad
H3K4me3 (Homo sapiens)
Method: dot blot assay
Attachment from submitter
compliant
Caption
Suitably modified peptides modeled on histone tails were obtained from Abcam. Each peptide is added to Bio-Dot apparatus at 0.01 and 0.1 ug / 50uL total volume (BioRad) Let sit on rocker 60 minutes. Pull through with vacuum, wash once with 100uL TBS and twice with 200uL TBSTw. Remove membrane from Bio-Dot and transfer to 10mL Pierce SuperBlock in tray. Place on rocker for 30 minutes. Dump off SuperBlock. Submitting lab has confirmed with the vendor that lots R0903B0054, DAM1619901, DAM1623866, DAM1644057, DAM1651667, and DAM1661080 are identical.
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad