ENCAB000BLB
Antibody against Homo sapiens H3K27me3
Homo sapiens
any cell type or tissue
not characterized to standards
- Status
- released
- Source (vendor)
- Millipore
- Product ID
- 07-449
- Lot ID
- JBC1854858(duplicated)
- Characterized targets
- H3K27me3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Aliases
- bradley-bernstein:PchAb 640(duplicated)
- External resources
Characterizations
H3K27me3 (Homo sapiens)
compliant
- Caption
- Suitably modified peptides modeled on histone tails were obtained from Abcam. Each peptide is added to Bio-Dot apparatus at 0.01 and 0.1 ug / 50uL total volume (BioRad). Let sit on rocker 60 minutes. Pull through with vacuum, wash once with 100uL TBS and twice with 200uL TBSTw. Remove membrane from Bio-Dot and transfer to 10mL Pierce SuperBlock in tray. Place on rocker for 30 minutes. Dump off SuperBlock. Added Primary Antibody in 10mL total volume. Placed on rocker for 30 minutes. Washed 3 x 5 minutes in TBSTw. Added secondary antibody diluted in SuperBlock in 10mL total volume. Placed on rocker for 30 minutes. Washed 3 x 15 minutes in TBSTw, 1 x 10 minutes in TBS. Imaged using ECL and a FluorChem Peltier-cooled camera (Cell Biosciences). The only peptide recognized by the H3K27me3 antibody was the correct one.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
H3K27me3 (Homo sapiens)
K562
not compliant
- Caption
- Western blot analysis of K562 lysate using rabbit polyclonal to H3K27me3. Submitting lab has confirmed with the vendor that lots 2039786, JBC1854858, JBC1873477.
- Submitter comment
- M Hatan reports lab confirmed with vendor that these lots are identical and should require only one characterization: 2039786, JBC1854858, JBC1873477
- Submitted by
- Meital Hatan
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991