ENCAB000BLB

Antibody against Homo sapiens H3K27me3

Homo sapiens
any cell type or tissue
not characterized to standards
Status
released
Source (vendor)
Millipore
Product ID
07-449
Lot ID
JBC1854858(duplicated)
Host
rabbit
Clonality
polyclonal
Aliases
bradley-bernstein:PchAb 640(duplicated)
External resources

Characterizations

H3K27me3 (Homo sapiens)
Method: dot blot assay
Attachment from submitter
compliant
Caption
Suitably modified peptides modeled on histone tails were obtained from Abcam. Each peptide is added to Bio-Dot apparatus at 0.01 and 0.1 ug / 50uL total volume (BioRad). Let sit on rocker 60 minutes. Pull through with vacuum, wash once with 100uL TBS and twice with 200uL TBSTw. Remove membrane from Bio-Dot and transfer to 10mL Pierce SuperBlock in tray. Place on rocker for 30 minutes. Dump off SuperBlock. Added Primary Antibody in 10mL total volume. Placed on rocker for 30 minutes. Washed 3 x 5 minutes in TBSTw. Added secondary antibody diluted in SuperBlock in 10mL total volume. Placed on rocker for 30 minutes. Washed 3 x 15 minutes in TBSTw, 1 x 10 minutes in TBS. Imaged using ECL and a FluorChem Peltier-cooled camera (Cell Biosciences). The only peptide recognized by the H3K27me3 antibody was the correct one.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
H3K27me3 (Homo sapiens)
K562
Method: immunoblot
Attachment from submitter
not compliant
Caption
Western blot analysis of K562 lysate using rabbit polyclonal to H3K27me3. Submitting lab has confirmed with the vendor that lots 2039786, JBC1854858, JBC1873477.
Submitter comment
M Hatan reports lab confirmed with vendor that these lots are identical and should require only one characterization: 2039786, JBC1854858, JBC1873477
Submitted by
Meital Hatan
Lab
Bradley Bernstein, Broad