ENCAB000BLA
Antibody against Homo sapiens H3K27me3
Homo sapiens
any cell type or tissue
characterized to standards
- Status
- released
- Source (vendor)
- Millipore
- Product ID
- 07-449
- Lot ID
- 2039786
- Characterized targets
- H3K27me3 (Homo sapiens)
- Lot ID aliases
- JBC1873477, JBC1854858
- Host
- rabbit
- Clonality
- polyclonal
- Aliases
- bradley-bernstein:PchAb 683, bradley-bernstein:PchAb 640, bradley-bernstein:PchAb 272
- External resources
Characterizations
H3K27me3 (Homo sapiens)
Method: dot blot assay
compliant
- Caption
- Suitably modified peptides modeled on histone tails were obtained from Abcam. Each peptide is added to Bio-Dot apparatus at 0.01 and 0.1 ug / 50uL total volume (BioRad). Let sit on rocker 60 minutes. Pull through with vacuum, wash once with 100uL TBS and twice with 200uL TBSTw. Remove membrane from Bio-Dot and transfer to 10mL Pierce SuperBlock in tray. Place on rocker for 30 minutes. Dump off SuperBlock. Added Primary Antibody in 10mL total volume. Placed on rocker for 30 minutes. Washed 3 x 5 minutes in TBSTw. Added secondary antibody diluted in SuperBlock in 10mL total volume. Placed on rocker for 30 minutes. Washed 3 x 15 minutes in TBSTw, 1 x 10 minutes in TBS. Imaged using ECL and a FluorChem Peltier-cooled camera (Cell Biosciences). The only peptide recognized by the H3K27me3 antibody was the correct one.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
H3K27me3 (Homo sapiens)
Method: dot blot assay
compliant
- Caption
- Suitably modified peptides modeled on histone tails were obtained from Abcam. Each peptide is added to Bio-Dot apparatus at 0.01 and 0.1 ug / 50uL total volume (BioRad). Let sit on rocker 60 minutes. Pull through with vacuum, wash once with 100uL TBS and twice with 200uL TBSTw. Remove membrane from Bio-Dot and transfer to 10mL Pierce SuperBlock in tray. Place on rocker for 30 minutes. Dump off SuperBlock. Added Primary Antibody in 10mL total volume. Placed on rocker for 30 minutes. Washed 3 x 5 minutes in TBSTw. Added secondary antibody diluted in SuperBlock in 10mL total volume. Placed on rocker for 30 minutes. Washed 3 x 15 minutes in TBSTw, 1 x 10 minutes in TBS. Imaged using ECL and a FluorChem Peltier-cooled camera (Cell Biosciences). The only peptide recognized by the H3K27me3 antibody was the correct one. according to the vendoe lot numbers
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
H3K27me3 (Homo sapiens)
Method: dot blot assay
not submitted for review by lab
- Caption
- This is a duplication of an earlier characterization that has already been reviewed.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
H3K27me3 (Homo sapiens)
Method: dot blot assay
compliant
- Caption
- Suitably modified peptides modeled on histone tails were obtained from Abcam. Each peptide is added to Bio-Dot apparatus at 0.01 and 0.1 ug / 50uL total volume (BioRad). Let sit on rocker 60 minutes. Pull through with vacuum, wash once with 100uL TBS and twice with 200uL TBSTw. Remove membrane from Bio-Dot and transfer to 10mL Pierce SuperBlock in tray. Place on rocker for 30 minutes. Dump off SuperBlock. Added Primary Antibody in 10mL total volume. Placed on rocker for 30 minutes. Washed 3 x 5 minutes in TBSTw. Added secondary antibody diluted in SuperBlock in 10mL total volume. Placed on rocker for 30 minutes. Washed 3 x 15 minutes in TBSTw, 1 x 10 minutes in TBS. Imaged using ECL and a FluorChem Peltier-cooled camera (Cell Biosciences). The only peptide recognized by the H3K27me3 antibody was the correct one.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
H3K27me3 (Homo sapiens)
K562
Method: immunoblot
compliant
- Caption
- Western blot analysis of K562 lysate using rabbit polyclonal to H3K27me3. The submitting lab has confirmed with the vendor that lots 2039786, JBC1854858, JBC1873477 are from the same physical stock.
- Submitter comment
- M Hatan reports lab confirmed with vendor that these lots are identical and should require only one characterization: 2039786, JBC1854858, JBC1873477
- Submitted by
- Meital Hatan
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991