ENCAB000BKU

Antibody against Homo sapiens H3K4me3

Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Diagenode
Product ID
pAb-003-050
Lot ID
A5051-001P
Host
rabbit
Clonality
polyclonal
Aliases
bradley-bernstein:PchAb 723
External resources

Characterizations

H3K4me3 (Homo sapiens)
K562
Method: immunoblot
Attachment from submitter
compliant
Caption
0.25ug unmodified Recombinant Histone H3 (Millipore 14-494) and 30ug of K562 Whole Cell Lysate (Abcam, ab7911) were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Panel A: anti Histone H3 (Abcam ab1791), band of expected size (~17kDa) visualized in both unmodified recombinant histone H3 and WCL, at similar intensities.Panel B: Band of expected size (~17kDa) visualized in WCL, and cannot be detected in unmodified recombinant Histone H3. Antibody seems to be specific since no reactivity with nonhistone proteins or unmodified histones was detected
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
H3K4me3 (Homo sapiens)
Method: dot blot assay
Attachment from submitter
compliant
Caption
Performed dot blot analysis using a large set of modified peptides. The only peptide recognized by the H3K4me3 antibody was the correct one.
Submitted by
Meital Hatan
Lab
Bradley Bernstein, Broad