ENCAB000BKR
Antibody against Homo sapiens H3K27ac
Bos taurus
any cell type or tissue
characterized to standards
Homo sapiens
any cell type or tissue
characterized to standards with exemption
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab4729
- Lot ID
- 34629
- Characterized targets
- H3K27ac (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Aliases
- bradley-bernstein:PchAb 170-V
- External resources
Characterizations
H3K27ac (Homo sapiens)
compliant
- Caption
- ChIP-ChIP comparison using a previously validated antibody. - Validation of ENCAB000BKR (ab4729 - 34629) as compared to ENCAB000AQN
- Submitter comment
- We used Pearson Correlation to compare the two tracks. Metrics were derived as follows: (1) Genomic windows for validation test: We collated a set of 15,157 3KB genomic windows whose ChIP-seq signals vary between histone marks and cell types, based on a manually curated set of ~1000 ChIP-seq experiments (data from the Encode2 and NIH Roadmap Epigenomics projects [URL here]). The genomic coordinates of these 15,157 3KB windows are available here. For the current validation exercise, we excluded 500 windows with false-positive constitutive signals across marks and input controls. (2) Antibody validation test: For each of the two ChIP-seq experiments, numerical values corresponding to normalized read density for each window were calculated. The Pearson correlation between both the two tracks was computed using the retained 14,657 genomic intervals and is reported above.
- Reviewer comment
- Both comparisons carried out in ACC112 cells, Pearson correlation of 0.9798
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
H3K27ac (Homo sapiens)
thymusthymus
compliant
- Submitter comment
- This western blot demonstrates the antibody recognizes a protein of the molecular weight consistent with human histone H3
- Reviewer comment
- Though this western blot was carried out with calf biosamples, the ENCODE Antibody Review Panel has reviewed this characterization and allowed this lot to be exempted for use in human biosamples on November 11, 2016.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991