ENCAB000BKR

Antibody against Homo sapiens H3K27ac

Bos taurus
any cell type or tissue
characterized to standards
Homo sapiens
any cell type or tissue
characterized to standards with exemption
Status
released
Source (vendor)
Abcam
Product ID
ab4729
Lot ID
34629
Characterized targets
H3K27ac (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Aliases
bradley-bernstein:PchAb 170-V
External resources

Characterizations

H3K27ac (Homo sapiens)
Method: ChIP-seq comparison
compliant
Caption
ChIP-ChIP comparison using a previously validated antibody. - Validation of ENCAB000BKR (ab4729 - 34629) as compared to ENCAB000AQN
Submitter comment
We used Pearson Correlation to compare the two tracks. Metrics were derived as follows: (1) Genomic windows for validation test: We collated a set of 15,157 3KB genomic windows whose ChIP-seq signals vary between histone marks and cell types, based on a manually curated set of ~1000 ChIP-seq experiments (data from the Encode2 and NIH Roadmap Epigenomics projects [URL here]). The genomic coordinates of these 15,157 3KB windows are available here. For the current validation exercise, we excluded 500 windows with false-positive constitutive signals across marks and input controls. (2) Antibody validation test: For each of the two ChIP-seq experiments, numerical values corresponding to normalized read density for each window were calculated. The Pearson correlation between both the two tracks was computed using the retained 14,657 genomic intervals and is reported above.
Reviewer comment
Both comparisons carried out in ACC112 cells, Pearson correlation of 0.9798
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
H3K27ac (Homo sapiens)
thymus
Method: immunoblot
compliant
Submitter comment
This western blot demonstrates the antibody recognizes a protein of the molecular weight consistent with human histone H3
Reviewer comment
Though this western blot was carried out with calf biosamples, the ENCODE Antibody Review Panel has reviewed this characterization and allowed this lot to be exempted for use in human biosamples on November 11, 2016.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad