ENCAB000BHY
Antibody against Homo sapiens NFIA
Homo sapiens
K562, HepG2
not characterized to standards
- Status
- released
- Source (vendor)
- Sigma
- Product ID
- WH0004774M2
- Lot ID
- 11161-1E11
- Characterized targets
- NFIA (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Isotype
- IgG1κ
- Antigen description
- NFIA (AAH22264, a.a. 1-499) full length recomb protein w/ GST tag. MW of GST tag is 26 kDa.
- External resources
Characterizations
NFIA (Homo sapiens)
K562HepG2
not compliant
- Caption
- An immunoprecipitation followed by Western blot was performed with Sigma WH0004774M2 (lot 11161-1E11) antibody against NFIA in K562 and HepG2 whole cell lysate along with a control IP with no lysate. The expected size of NFIA is 60 kDa. A band of ~60 kDa was detected in the test IP and not in the control IP. The experiment was repeated and the band was analyzed by mass spec in the secondary validation.
- Submitter comment
- no IgG control
- Reviewer comment
- Missing IgG control
- Submitted by
- Flo Pauli-Behn
- Lab
- Richard Myers, HAIB
- Grant
- U54HG006998