ENCAB000BHY

Antibody against Homo sapiens NFIA

Homo sapiens
K562, HepG2
not characterized to standards
Status
released
Source (vendor)
Sigma
Product ID
WH0004774M2
Lot ID
11161-1E11
Characterized targets
NFIA (Homo sapiens)
Host
mouse
Clonality
monoclonal
Isotype
IgG1κ
Antigen description
NFIA (AAH22264, a.a. 1-499) full length recomb protein w/ GST tag. MW of GST tag is 26 kDa.
External resources

Characterizations

NFIA (Homo sapiens)
K562HepG2
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
An immunoprecipitation followed by Western blot was performed with Sigma WH0004774M2 (lot 11161-1E11) antibody against NFIA in K562 and HepG2 whole cell lysate along with a control IP with no lysate. The expected size of NFIA is 60 kDa. A band of ~60 kDa was detected in the test IP and not in the control IP. The experiment was repeated and the band was analyzed by mass spec in the secondary validation.
Submitter comment
no IgG control
Reviewer comment
Missing IgG control
Submitted by
Flo Pauli-Behn
Lab
Richard Myers, HAIB