ENCAB000BFY

Antibody against Homo sapiens PCBP2

Homo sapiens
HepG2, K562
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
MBLI
Product ID
RN025P
Lot ID
001
Characterized targets
PCBP2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
KLH conjugated synthetic peptide, corresponding to internal region of human PCBP2
Aliases
xiang-dong-fu:PCBP2
External resources

Characterizations

PCBP2 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using PCBP2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PCBP2 antibody (lanes under 'PCBP2').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
PCBP2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using PCBP2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PCBP2 antibody (lanes under 'PCBP2').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
PCBP2 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
IP-WB characterization of PCBP2 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-PCBP2(Human)pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
PCBP2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against PCBP2 in HepG2 whole cell lysate using PCBP2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PCBP2. PCBP2 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
PCBP2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against PCBP2 in K562 whole cell lysate using PCBP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PCBP2. PCBP2 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn