ENCAB000BFN
Antibody against Homo sapiens HNRNPK
Homo sapiens
K562, HepG2
characterized to standards
- Status
- released
- Source (vendor)
- MBLI
- Product ID
- RN019P
- Lot ID
- 001
- Characterized targets
- HNRNPK (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- KLH conjugated synthetic peptide, corresponding to C-terminus of human HNRNPK
- Aliases
- xiang-dong-fu:HNRNPK
- External resources
Characterizations
HNRNPK (Homo sapiens)
HepG2
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPK specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-HNRNPK antibody (lanes under 'HNRNPK').
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- HEPG2_MBL_RN019P_1_HNRNPK.png
HNRNPK (Homo sapiens)
compliant
- Caption
- Western blot following shRNA against HNRNPK in K562 and HepG2 whole cell lysate using HNRNPK specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HNRNPK. Lanes 5-7 follow the same pattern, but in HepG2. HNRNPK protein appears as the green band, GAPDH serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- HNRNPK_Secondary_Western.png
HNRNPK (Homo sapiens)
K562
compliant
- Caption
- IP-WB characterization of HNRNPK specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-HNRNPK(Human)pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN019P_001_HNRNPK_K562.png