1. Antibodies
  2. Homo sapiens
  3. RB1

ENCAB000BFB

Antibody against Homo sapiens RB1

Homo sapiens
GM12878, K562, HepG2
characterized to standards
Homo sapiens
any cell type or tissue, HEK293T
partially characterized
Homo sapiens
HeLa-S3, IMR-90, heart
not characterized to standards
Status
released
Source (vendor)
Cell Signaling
Product ID
9313S
Lot ID
2
Characterized targets
RB1 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide corresponding to central residues of mouse Rb.
External resources

Characterizations

RB1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody 9313S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 110.0
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
RB1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody 9313S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 110.0
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
RB1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody 9313S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 110.0
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
 expt1032-6.jpg
RB1 (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T using the antibody 9313S. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 110.0.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
RB1 (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90heart
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, HepG2, IMR90 and Heart using the antibody 9313S. Molecular mass: 106159 Da
Reviewer comment
Band is either missing (lanes 3 and 6) or is not 50% of signal (lanes 2 and 5). Lanes 1 and 4 are compliant.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
RB1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody 9313S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~106 kDa.
Reviewer comment
Multiple bands but major band within 20% of expected size and >50% of total signal in the lane.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
RB1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 whole cell lysates using 9313S, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was silver-stained, gel fragments corresponding to the bands indicated were excised and destained using the SilverSNAP Stain for Mass Spectrometry (Pierce). Then proteins were treated with trypsin using the in-gel digestion method. Digested proteins were analyzed on a LTQ-Orbitrap (Thermo Scientific) b the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (protein false discovery rate <1%, 2 peptides per protein minimum).
Reviewer comment
Need to provide full peptide list.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
RB1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of Rb from K562 cells using 9313S. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with 9313S. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. Expected band size ~110 kD.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download
 Rb_IP.png