ENCAB000BEH

Antibody against Homo sapiens ARID3A

Homo sapiens
GM12878, K562, HepG2, HeLa-S3, liver
not characterized to standards
Status
released
Source (vendor)
CDI
Product ID
R21.1.1E9
Lot ID
100212.SVG
Characterized targets
ARID3A (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
affinity
Isotype
IgG1
External resources

Characterizations

ARID3A (Homo sapiens)
GM12878K562HepG2HeLa-S3liver
Method: immunoblot
Attachment from submitter
not compliant
Caption
a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3, Liver using the antibody R21.1.1E9.
Reviewer comment
Multiple bands, none >50% of total signal in lane. Expected size (~63kD) not indicated.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
ARID3A (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody R21.1.1E9 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Multiple bands - may possibly be rescued with a knockdown against the factor.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford