ENCAB000BEB

Antibody against Homo sapiens SUZ12

Homo sapiens
IMR-90, MCF-7, HEK293T, heart, HeLa-S3, K562, HepG2, GM12878
characterized to standards
Status
released
Source (vendor)
Cell Signaling
Product ID
3737S
Lot ID
3
Characterized targets
SUZ12 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide corresponding to the sequence of the human SUZ12 protein.
Aliases
bradley-bernstein:PchAb 1216
External resources

Characterizations

SUZ12 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody 3737S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
SUZ12 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody 3737S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected Molecular Weight: 83 KD
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
SUZ12 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody 3737S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
SUZ12 (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T using the antibody 3737S. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 83.0.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
SUZ12 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody 3737S. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 83.0.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
SUZ12 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody 3737S, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
SUZ12 is component of the PRC2/EED-EZH2 complex, http://www.genecards.org/cgi-bin/carddisp.pl?gene=SUZ12&keywords=suz12. SUZ12 and EZH2 has interaction, 27 publications , http://thebiogrid.org/117059/summary/homo-sapiens/suz12.html. SUZ12 has interaction with PHF19, 4 publications, http://thebiogrid.org/117059/summary/homo-sapiens/suz12.html.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
SUZ12 (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90heart
Method: immunoblot
Attachment from submitter
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, HepG2, IMR90 and Heart using the antibody 3737S. Molecular mass: 83055 Da
Reviewer comment
No expected MW size given. Also there's 6 lanes but 7 shown in the caption.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
SUZ12 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562 using the antibody 3737S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~ 83 kDa
Reviewer comment
Expected MW size not given.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
SUZ12 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
K562 whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction along side a whole cell lysate were loaded on a CriterionXT gel and separated. After separation, the samples were transferred using a wet transfer. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed. Band of expected size visualized representing strongest signal in the lane.
Reviewer comment
no IgG shown
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad