1. Antibodies
  2. Homo sapiens
  3. RNF2

ENCAB000BEA

Antibody against Homo sapiens RNF2

Homo sapiens
K562, HeLa-S3, HepG2, IMR-90, MCF-7
characterized to standards
Homo sapiens
GM12878, any cell type or tissue
partially characterized
Homo sapiens
heart
not characterized to standards
Status
released
Source (vendor)
Cell Signaling
Product ID
5694S
Lot ID
1
Characterized targets
RNF2 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Purification
affinity
Isotype
IgG
Antigen description
The full-length human RING1B protein.
External resources

Characterizations

RNF2 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody 5694S. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 41.0.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
RNF2 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody 5694S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 41.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
RNF2 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody 5694S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
 expt1032_2.jpg
RNF2 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 whole cell lysates using 5694S, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was silver-stained, gel fragments corresponding to the bands indicated were excised and destained using the SilverSNAP Stain for Mass Spectrometry (Pierce). Then proteins were treated with trypsin using the in-gel digestion method. Digested proteins were analyzed on a LTQ-Orbitrap (Thermo Scientific) b the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (protein false discovery rate <1%, 2 peptides per protein minimum).
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
RNF2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of RING1b from K562 cells using 5694S. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with 5694S. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. Expected band size ~41 kD.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download
 RING1B_IP.png
RNF2 (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90heart
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, HepG2, IMR90 and Heart using the antibody 5694S. Molecular mass: 37655 Da
Reviewer comment
Band is either missing (lane 6) or is not 50% of signal (lane 1). Lanes 2, 3, 4, and 5 are compliant.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
RNF2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody 5694S .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~37 kDa.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford